Unit code: HES4641
|Credit points||12.5 Credit Points|
|Contact hours||48 Hours|
Related course(s)A unit of study in the Bachelor of Science (Biochemistry/Chemistry), Bachelor of Science (Biotechnology),
Bachelor of Science (Biotechnology)/Bachelor of Arts (Media and Communications), Bachelor of Science (Biotechnology)/Bachelor of Business and Bachelor of Science (Psychology/Biochemistry).
Aims and objectives
The first aim is to have students become competent in the basic experimental techniques of biochemistry and molecular biology including use of vertical and flat-bed gel apparatus, spectrophotometers with data acquisition and analysis by computer, spectrofluorimetry, and various centrifuges including the ultracentrifuge. Students work in pairs with minimal supervision and are encouraged to solve their own problems of technique. The second aim is to have students become competent in data organisation, presentation and report writing.
At the end of this unit of study students will be able to
- Analyse and assess particular separation processes.
- Describe particular applications of biotechnology.
- Be able to answer questions relating to biotechnology industry practices and standards.
- Be able to answer questions and make predictions based on knowledge of immunological and immunochemical technology.
- Be able to include DNA array technology applications in project design.
Generic skills outcomes
Students are expected to enhance several of their graduate attributes during this unit of study and should consult with your lecturer if not clear as to how this unit of study achieves this. The graduate attributes which relate to this unit of study help to produce students who:
Are capable in their chosen professional, vocational or study areas.
- Have a basic understanding of the theoretical principles involved in the study area.
- Have an in-depth technical competence in the specific (core) discipline.
- Can apply specific knowledge of the (core) discipline to real situations.
- Have the ability to engage in critical inquiry.
Operate effectively and ethically in work and community situations.
- Have the ability to work both independently and collaboratively.
- Can effectively communicate within and without the subject discipline.
Are aware of environments in which they will be contributing.
- Have a broad understanding of the need for sustainability and sustainable development.
- Have a basic understanding of the need to carry out work in an ethical and socially responsible fashion.
IEAust Generic Attributes
In addition to the Swinburne generic attributes, this unit is also expected to enhance skills recognised by the Institution of Engineers (Australia), specifically:
- Ability to apply knowledge of basic science and engineering fundamentals.
- Ability to communicate effectively, not only with engineers but also with the community at large.
- In-depth technical competence in at least one engineering discipline.
- Ability to utilise a systems approach to design and operational performance.
- Understanding of the social, cultural, global and environmental responsibilities of the professional engineer, and the need for sustainable development.
- Understanding of the principles of sustainable design and development.
ContentA number of experimental exercises, including sulphydryl analysis, peptide sequencing, gel electrophoresis of proteins and nucleic acids, preparation and analysis of plasmid DNA, cell fractionation and enzyme assays. Instrumentation used include centrifuges, electrophoresis apparatus, spectrophotometer, spectrofluorimeter, image capture devices.
Reading materialsA current Practical Manual must be purchased from the Bookshop.
A selection of research papers are cited in the practical manual.
Selected reading on Library Counter Reserve and electronic reserve.
Selected web resources